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It inhibits the ligation activity.
Antimicrobial agents interfere with either DNA replication cell wall synthesis, plasma membrane integrity, ribosomal function, or folate synthesis. Ciprofloxacin belongs to fluoroquinolones class of antibiotics that interfere with DNA replication. DNA gyrase is a bacterial topoisomerase that cuts and then reseals DNA to change DNA topology during replication. The binding of ciprofloxacin to A subunit of gyrase enzyme stabilizing gyrase–DNA complex. This leads to inhibition the resealing (ligation) activity of the enzyme leaving the DNA fragmented.
