<span>Maintaining a patent airway</span>
The correct answer is four base pairs.
BamHI is endonuclease meaning that it has the ability to cut DNA at specific target site. The recognition sequence of BamHI is 5'-GGATCC-3 and it cleaves it after the first G. Sticky ends are formed as a result of that cleavage remaining a four-base 5' overhang in one molecule and a complementary 5' overhang in the other. These ends are can be joined back together by an enzyme called ligase.
During Prophase chromosomes condense, and mitotic spindle form, chromosomes are copied, and the nuclear membrane disappears, spindle fibres pull the sister chromatids apart.
Explanation:
Prophase is divided into 2 sub-phases as early Prophase and late prophase.
Early Prophase:
In early prophase distinct thick chromosome like structures, Centrioles move to the opposite poles and, nuclear membrane disappears
By late prophase:
Astral rays and spindle fibres are formed. Spindle fibres attach to the chromosome. Contractions occur in the attachment and sister chromatids are pull apart towards the equator.
Metaphase will follow the prophase.