1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Slav-nsk [51]
2 years ago
13

A scientist runs an SDS-PAGE experiment two separate times on a protein with a molecular weight of 200 kD. For the first experim

ent he treats the protein with SDS only and for the second experiment he treats the protein with both SDS and a reducing agent. If the first gel yields one band showing a molecular weight of 200 kD and the second run yields one band showing a molecular weight of 25 kD, the researcher should conclude that
Biology
1 answer:
Delicious77 [7]2 years ago
3 0

Answer:

This protein consists of 8 subunits of equal size linked by disulfide bonds

Explanation:

The sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) is a technique widely used in molecular biology laboratories to separate proteins with molecular weights between 5 and 250 kDa. SDS is an anionic detergent used to denature proteins before electrophoresis. SDS can denature proteins by altering non-covalent bonds such as hydrogen, hydrophobic and ionic interactions, but they cannot cleave disulfide bonds. In this case, reducing agents (e.g.,  β-mercaptoethanol or dithiothreitol) have been used to cleave disulfide bonds.

You might be interested in
una de las hebras de ADN de un cromosoma de cierto organismo tiene 5 millones de bases nitrogenadas ¿cuantos nucleótidos debe te
avanturin [10]
La hebra complementaria debe tener 5 millones de nucleótidos, porque cada nucleótido tiene una base nitrogenada, y la hebra complementaria debe tener la misma cantidad de bases nitrogenadas que la hebra original. (Recuerda que las dos hebras "encajan" una con otra).


Ver más en Brainly.lat - https://brainly.lat/tarea/8051246#readmore
6 0
3 years ago
An increase in the volume of forests and phytoplankton would lead to a reduction in free "blank" , which in turn could cause a d
lord [1]

Answer: Carbon dioxide

Explanation:

The forests and phytoplankton are carbon sinks, which absorbs the green house gas carbon dioxide from the atmosphere for photosynthesis. The carbon dioxide from the atmosphere and water are used as reactant in photosynthesis to yield carbohydrates and oxygen as products. Thus the carbon dioxide level in atmosphere decreases. This is necessary for controlling pollution in the environment and reducing the environmental temperature as carbon dioxide can make the environment warm.

5 0
2 years ago
Please help me with these two questions I don’t Know these two ill mark you as the best answer
Kobotan [32]
I’m sorry if I get it wrong but I think it’s C for the first one and B for the second one
7 0
3 years ago
If a population is at Hardy-Weinberg equilibrium...
jarptica [38.1K]

Answer:

B. (i) yes; (ii)-yes

Explanation:

If a population is at Hardy-Weinberg equilibrium, allele frequencies can be calculated from the given values of genotype frequencies and genotype frequencies can be calculated if the allele frequencies are given.

For a population in Hardy-Weinberg equilibrium; p+q=1

Here, p= frequency of recessive allele and q = frequency of dominant allele.

And p2 + 2pq + q2 = 1

p2= Frequency of homozygous dominant genotype

2pq = Frequency of heterozygous dominant genotype

q2 = Frequency of homozygous recessive genotype

For example, if frequency of homozygous recessive genotype (q2) is 0.40, then, the frequency of recessive allele (q) will be = square root of 0.40= 0.63

Likewise, frequency of dominant allele (p) in this population will be = 1-q = 1-0.63 = 0.37

3 0
3 years ago
Which of the following is not considered a criterion that suggests the presence of a psychological disorder under the category o
mojhsa [17]
<h2>The correct answer is (A)</h2>

Explanation:

  • Maladaptiveness is a condition when the person's ability is inhibited to adjust to situations or conditions.
  • Personal unhappiness do not come under the category of Maladptiveness.
  • The other features such as impaired functioning, long periods of discomfort and disruptive behavior comes under the category of Maladaptiveness.
  • The cause of Maladaptive behavior can be major change in lifestyle or due to chronic illness.
  • In Maladaptive behavior a person is not able to see a path for the future he desired.

3 0
3 years ago
Other questions:
  • When molecules have an uneven distribution of charges they are said to be
    15·1 answer
  • Which of the following is natural gas used for in the United States?
    15·2 answers
  • Paramecium caudatum uses its cilia to _____.
    11·2 answers
  • What is a monosynaptic reflex arc?
    9·1 answer
  • Which of the following is not attached to the central carbon atom in an amino acid?
    5·1 answer
  • Please I need help with question 35 and 36 and it’s very hard and I’m struggling with it and if you need to see the picture big
    9·2 answers
  • After playing in an old shed, two boys tell their mother that they hurt all over. She checks and finds that they both have fever
    15·1 answer
  • MARKING PEOPLE AS BRAINLIST ⚠️⚠️⚠️<br><br> Give three pros and cons of fire suppression
    5·1 answer
  • An acid is best defined as: Select one:
    15·2 answers
  • Exam number: 986210RR
    5·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!