Answer:
tetracycline
Explanation:
When the DNA insert is ligated in Pstl site, the ampicillin gene is disrupted while <em>tetracycline gene is still intact</em>.
- If the bacterial colony is growth in a medium with only ampicillin, only the cells that contains the plasmid with the insert will grow.
- If the bacterial colony is growth in a medium with only tetracycline, the cells which have incorporated the plasmid will survive. We cannot distinguish between cells with plasmid + insert and plasmid alone.
- If the bacterial colony is growth in a plate with ampicillin + tetracyclin, only the cells with the plasmid without the insert will grow.
Therefore, to ensure that the cells of a bacterial colony contains the plasmid, they have to be seeded in a plate with tetracycline.
Base, alkali...something like that
Several factors affect the rate at which enzymatic reactions proceed - temperature,pH<span>, enzyme </span>concentration<span>, </span>substrate concentration<span>, and the presence of any inhibitors or activators.</span>
The correct answer is "<span>the presence of the appropriate receptors on the cells of the target tissue or organ".
Hormones are chemical messengers that work to modify the target cell's actions. Hormones work by attaching to its appropriate receptor, then the receptor will be activated and signal transduction will take place. Signal transduction mechanisms will eventually activate a protein kinase which will phosphorylate a certain protein (usually a promoter for transcription) which will then start the production of a certain protein with a specific function.
For instance, insulin is a hormone. Insulin will then bind to muscle and adipose cells which will activate signal transduction mechanisms that will eventually lead to the translation of the glucose receptor and channel proteins which will increase the uptake of glucose in the blood stream and lower the blood glucose levels.</span>
