A
B is wrong because mitochondria would likely absorb the stain even if it was dead.(And most cells that have been treated are dead anyway). C and D are just throwaway answers, you usually want to avoid the word "always" when drawing conclusions.
Answer:
Answer B. Endosymbiosis
Explanation:
Early protists were first eukaryotic organisms and had several membrane bound organelles. The anaerobic protists evolved by acquiring purple bacteria. The purple bacteria have the ability to carry out aerobic cellular respiration. The endosymbiont transformed into mitochondria in due course of evolution. Similarly, the photosynthesizing protists were evolved by endosymbiosis of cyanobacteria which in turn was transformed into chloroplast during further evolution.
nucleus is the structure responsible.