Facilitated diffusion does not use cellular energy.
Since the transportation of molecules occurs through the concentration gradient, it doesn’t use cellular energy for transportation of molecules.
DNA ligase is the enzyme that repair irregularities and breaks in the back bone of double stranded DNA. The enzyme function by sealing the repair in the DNA, it seals recombination fragments and it connects okazaki fragments during replication of DNA.
If a mutation reduce the amount of ligase, all the functions of the ligase enzyme listed above will be affected negatively.
