Answer:Carbon dioxide molecules
Explanation:
When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
" Gametophyte, Secondary. "
Answer:
Cellular respiration that proceeds in the absence of oxygen is anaerobic respiration. Cellular respiration that proceeds in the presence of oxygen is aerobic respiration. Anaerobic respiration evolved prior to aerobic respiration.