Answer:
dear user the answer is option A.oxygen level and water temperature and C.salinity and water temperature.
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There is a place in the lid where the fluid rushes to when you tip it, leaving the air bubbles in the cylinder of the bottle. In some companies it's a chemical inside the fluid that can evaporate over time and looses it's " magic" effect.
Isotonic since in a hypertonic solution the cell would shrivel up and in a hypotonic solution it would lyse (burst).
