Answer:
Measure 2.47 mL of the stock solution (i.e 0.0405 mM) and dilute it to the 100 mL mark with water
Explanation:
To make 100 mL of 0.001 mM solution from 0.0405mM solution, we need to determine the volume of 0.0405mM solution needed. This can be obtained as follow:
Molarity of stock (M₁) = 0.0405 mM
Volume of diluted (V₂) = 100 mL
Molarity of diluted solution (M₂) = 0.001 mM
Volume of stock solution needed (V₁) =?
M₁V₁ = M₂V₂
0.0405 × V₁ = 0.001 × 100
0.0405 × V₁ = 0.1
Divide both side by 0.0405
V₁ = 0.1 / 0.0405
V₁ = 2.47 mL
Therefore, to make 100 mL of 0.001 mM solution from 0.0405mM solution, measure 2.47 mL of the stock solution (i.e 0.0405 mM) and dilute it to the 100 mL mark with water.
