Rattle snake venom is cytotoxin
Rattle snake venom is protein
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Earthworms, because fishes bodies would just be eroded by the water
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The animals are used for examining and determining the effect of a particular new drug for the treatment of the diseases and disorders before they are examined over humans. This practice is called as clinical trial.
The ethical issue associated with given type of testing is that animals have right to live and survive. They should not be subjected to the treatment where they experience harm or even death.