Which one do you want answered
        
                    
             
        
        
        
Nalah could use only 1 rat and test it multiple times.
        
             
        
        
        
Yes they can Only eukaryotes form multicellular organisms consisting of many kinds of tissue made up of different cell types
        
             
        
        
        
Answer:
First, you need to retrieve the FASTA sequences of the already sequenced genes. After that, you should make a sequence alignment on a software like CLUSTALW (https://www.ebi.ac.uk/Tools/msa/clustalw2/) to seek for the conserved regions of the gene. Based on this regions, you will design your FISH probe, which for bacteria can start in 33 bp (Ming Tan <em>et al.</em>, 2019), but a 200 bp probe is a very suitable size. There are several companies that fabricates this custom probes, and sell all the reagents to perform the experiments. Having this, you will perform your FISH experiment to see which cells are actively degrading Toluene.
Explanation: