Answer:
10% and Uremia
Explanation:
The kidneys are able to perform their homeostatic functions reasonably well as long as at least 10% of the nephrons are functioning normally. If fewer nephrons are functioning, uremia , or toxic buildup of waste products in the blood, is possible.
Inner core, outer core, mantle, crust
The appropriate sequence of steps involved in recombinant DNA technology are as under :-
1) Selected gene is isolated.
2) Restriction enzymes cut DNA into fragments.
3) Fragments of DNA are inserted into a vector.
4) Vector and recombinant DNA multiply.
5) Recombinant DNA is inserted into host.
In the very first step, the desired gene/genetic material for cloning is isolated from the cell. To do this the cell is lysed. Cells can be lysed by sonication or using chemicals like detergents.
In the second step, isolated DNA/gene is cut with the help pf biological scalpels known as restriction enzymes. The vector (usually it is a plasmid) is also cut down using restriction enzymes.
In the third step, the fragment of the restriction digested DNA (gene) and the vector are ligated together.
In the fourth step, this vector in which desired gene has been incorporated is cloned to generate various copies.
In the last step, this recombinant DNA is inserted into a host cell where is expressed i.e. in host cell this desired gene produces its product (protein).
<span>Both the male and female gametes are formed during a process of cellular reproduction called meiosis. During meiosis the DNA is only replicated or copied 1 time. However, the cells are divided into 4 separate cells. This means that the new gamete cells have only half of the number of chromosomes as other cells.</span>