Single-cell RNA-sequencing (scRNA-seq) provides the chance to analyze heterogeneous cellular compositions and probe the patterns of gene expression that are unique to each cell type under various circumstances. However, batch effects like lab setups and individual variability make it difficult to use them in cross-condition designs.
<h3>What is Single-cell transcriptomes ?</h3>
In single-cell transcriptomics, the messenger RNA levels of hundreds to thousands of genes are simultaneously measured to assess the degree of gene expression in individual cells within a particular population.
<h3>Advantages : </h3>
• Integrated protocol proceeds directly from whole cells and preserves sample integrity.
• High resolution analysis enables discovery of cellular differences typically hidden by bulk sampling methods.
• Robust transcriptome analysis down to single-cell input levels for high-quality samples.
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Messenger RNA (mRNA) molecules carry the coding sequences for protein synthesis and are called transcripts; ribosomal RNA (rRNA) molecules form the core of a cell's ribosomes (the structures in which protein synthesis takes place); and transfer RNA (tRNA) molecules carry amino acids to the ribosomes during protein synthesis. In eukaryotic cells, each class of RNA has its own polymerase, whereas in prokaryotic cells, a single RNA polymerase synthesizes the different class of RNA. Other types of RNA also exist but are not as well understood, although they appear to play regulatory roles in gene expression and also be involved in protection against invading viruses.
Explanation:
In digestion, the liver is involved in processing the nutrients that are absorbed from the small intestine. It helps in the digestion of fat.
On the other hand, the liver maintains homeostasis by serving an important function in the excretory system.
