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Paraphin [41]
2 years ago
7

Instead of thymine, RNA Polymerase pairs the nucleotide base __________ with adenine.

Biology
1 answer:
Luba_88 [7]2 years ago
7 0

Answer:

Uracil

Explanation:

DNA and RNA are both nucleic acids with similar structures, but they have a few key differences.

RNA is primarily single stranded, whereas DNA forms a double-stranded helix.

The sugar backbone in a DNA molecule is deoxyribose, whereas in RNA it is ribose.

The nucleotides that make up DNA molecules are adenine, thymine, cytosine, and guanine, whereas nucleotides that make up RNA are slightly different, adenine, uracil, cytosine, and guanine.

In DNA, adenine pairs with thymine, and guanine pairs with cytosine.

In RNA, adenine pairs with uracil, and guanine pairs with cytosine.

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(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.

To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.

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