The red-legged frog breeds in ephemeral ponds from January through March. Its relative, the bullfrog, breeds in permanent ponds from late March through May. There are two pre-mating isolation mechanism at play here,
1. Ecological isolation: The habitats are different, hence the individuals of both the species do not meet. Ephemeral ponds are temporary ponds that develop during rainy days, while the permanent ponds are full of water throughout the year.
2. Temporal isolation: The time of mating is different for both the species to avoid contact between the individuals of closely related species. Red-legged frogs mate in January to March slot and the bullfrog in the March to May slot.
In prophase 1:
Chromosomes become visible, crossing-over occurs, the nucleolus disappears, the meiotic spindle forms, and the nuclear envelope disappears.
In metaphase 1:
The pairs of chromosomes (bivalents) become arranged on the metaphase plate and are attached to the now fully formed meiotic spindle. The centrioles are at opposite poles of the cell.
Student 1’s methods would be more accurate, because the student would control more factors. Only one variable at a time (either temperature or acidity) would be tested on each group of worms. On the other hand, Student 2 is testing both factors on all the worms, which could make the results unclear.