Answer:
RFLP analysis.
Explanation:
RFLP (Restriction fragment length polymorphism ) may be defined as a molecular technique used to determine the location of a particular gene in the DNA sequences. The individuals among the population can be easily identified by RFLP analysis.
The restriction enzyme is used for the digestion of DNA sample. The restriction fragments are then visualized by running the fragments on gel electrophoresis. The hybridization between single stranded nucleic acid is not involved in the RFLP.
Thus, the correct answer is option (1).
2nd choice is the correct one
Answer:
0.0177
Explanation:
Cystic fibrosis is an autosomal recessive disease, thereby an individual must have both copies of the CFTR mutant alleles to have this disease. The Hardy-Weinberg equilibrium states that p² + 2pq + q² = 1, where p² represents the frequency of the homo-zygous dominant genotype (normal phenotype), q² represents the frequency of the homo-zygous recessive genotype (cystic fibrosis phenotype), and 2pq represents the frequency of the heterozygous genotype (individuals that carry one copy of the CFTR mutant allele). Moreover, under Hardy-Weinberg equilibrium, the sum of the dominant 'p' allele frequency and the recessive 'q' allele frequency is equal to 1. In this case, we can observe that the frequency of the homo-zygous recessive condition for cystic fibrosis (q²) is 1/3200. In consequence, the frequency of the recessive allele for cystic fibrosis can be calculated as follows:
1/3200 = q² (have two CFTR mutant alleles) >>
q = √ (1/3200) = 1/56.57 >>
- Frequency of the CFTR allele q = 1/56.57 = 0.0177
- Frequency of the dominant 'normal' allele p = 1 - q = 1 - 0.0177 = 0.9823
Answer: D. The genes for antibiotic resistance help the transformed bacteria survive in their environment.
Explanation:
First and foremost, we should note that plasmids used in the delivery of DNA typically consists of genes which helps in antibiotic resistance.
Based on the question given, we should note that the genes for antibiotic resistance help the transformed bacteria survive in their environment. This is because cells that have been treated with plasmid are able to survive and grow compared to those that have not been treated with plasmid which are eventually killed by the antibiotic.
