In a circular bacterial chromosome, the structure of DNA is a right-handed double helix In a circular bacterial chromosome.
If DNA is twisted in the right-handed direction, it becomes overwound. Overwinding results in positive supercoiling takes place. If DNA is twisted in the left handd direction, it becomes underwound. Underwinding results in negative supercoiling.
f. One effect negative supercoiling in bacterial chromosomes is to promote separation of the two strands of DNA in the double helix
