Non-fluorescent calcein AM is hydrolyzed by intracellular esterases into the green fluorescent dye calcein, which can be used to quantify the number of viable cells.
https://www.creative-bioarray.com/support/calcein-am-cell-viability-assay.htm
The answer is True.
Physical changes are used to separate mixtures into their component compounds, but can not usually be used to separate compounds into chemical elements or simpler compounds. Physical changes occur when objects or substances undergo a change that does not change their chemical composition.
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