Answer:
I think it should be C and D both.
<span>d. Disk soaked in distilled water
Experimentation is a scientific process wherein you test and verify a hypothesis based on its assumption is going to be supported or negated (null hypothesis). Hence experimentation involves the independent and dependent variable, also control variable as a level in the IV. </span>
A replication bubble forms as the DNA unwinds.
Option C
<u>Explanation</u>:
Replication bubble is the space where the strands of double helical DNA structure are separated with the help of DNA helicase. The strands of the double helical DNA are still in the helix form on the both end of the unwinded DNA stand which gives this region an appearance of a bubble.
DNA unwinding is the first step in replication or transcription of the DNA molecule. DNA helicase separates the two types of "DNA" that are connected by hydrogen bonds with the help of ATP hydrolysis.
<span>Lafora disease is the most severe teenage-onset progressive epilepsy, a unique form of glycogenosis with perikaryal accumulation of an abnormal form of glycogen, and a neurodegenerative disorder exhibiting an unusual generalized organellar disintegration. The disease is caused by mutations of the EPM2A gene, which encodes two isoforms of the laforin protein tyrosine phosphatase, having alternate carboxyl termini, one localized in the cytoplasm (endoplasmic reticulum) and the other in the nucleus. To date, all documented disease mutations, including the knockout mouse model deletion, have been in the segment of the protein common to both isoforms. It is therefore not known whether dysfunction of the cytoplasmic, nuclear, or both isoforms leads to the disease. In the present work, we identify six novel mutations, one of which, c.950insT (Q319fs), is the first mutation specific to the cytoplasmic laforin isoform, implicating this isoform in disease pathogenesis. To confirm this mutation's deleterious effect on laforin, we studied the resultant protein's subcellular localization and function and show a drastic reduction in its phosphatase activity, despite maintenance of its location at the endoplasmic reticulum.
I got my information from </span>https://www.ncbi.nlm.nih.gov/pubmed/14722920
When the cells undergo late apoptosis, the membrane structure is destroyed and the nuclear structure can be selectively visualized by Hoechst 33342/PI.
https://www.creative-bioarray.com/support/double-staining-apoptosis-assay-hoechst33342-pi.htm
