blue......................
Simple version:
First, the section with the desirable gene must be identified. Assuming that has already happened, the section of DNA must be excised from the original genome using restriction enzymes, which recognize certain DNA sequences and snip DNA at those sites. DNA ligase is used to "glue" these ends back together. The DNA is inserted into a plasmid (also with restriction enzymes), which would usually contain antibiotic-resistance genes (so they survive in an environment containing the antibiotic, which would also help show if the bacteria have been successfully transformed).
Then comes the actual transformation process. The bacteria to be transformed are mixed with calcium chloride (which causes the bacteria to be more receptive to the plasmids) and then mixed with the plasmids. The bacterial cells are subjected to a heat shock (the solution is heated and rapidly cooled, e.g. by placing the mixture in a hot water bath and quickly transferred to ice) so they will take up the plasmid (since the temperature change makes the membrane more permeable). The bacteria are placed on a growth medium containing the antibiotic they're resistant to. Only those successfully transformed would survive.
mutation is the permanent alteration of the nucleotide sequence of the genome of an organism, virus, or extrachromosomal DNA or other genetic elementsit is of two types :- point mutation and frame shift mutation
point mutation - mutation only in a single base
frame shift - in the entire sequence or an entire set
Answer:
Plasma.
Explanation:
The main connective tissue of human body is blood. Blood mainly composed of the plasma and formed elements. Formed elements include white blood cell, red blood cells and platelets.
Blood plasma is present around 55% in composition. The blood plasma contains various coagulant factors, ions, proteins and chemicals. Plasma is the fluid portion of blood that needs to be drawn in presence of the anticoagulant compound.
Thus, the answer is plasma.
