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Alex777 [14]
3 years ago
10

SIMPLE STAINING OF BACTERIA AND YEAST

Biology
1 answer:
grin007 [14]3 years ago
6 0
For staining flagella of bacteria use actively motile organisms 20 to 24 hours old, allow to diffuse in sterile water 20 to 30 minutes, transfer droplets of the suspension to clean slides and let evaporate without spreading. Then treat 2 to 4 minutes with the following mordant: tannic acid 10 or 20%, 50 cc.; ferric chloride 5%, 10 to 15 cc.; carbol fuchsin (Ziehl-Nielson), 5 cc.; hydrogen peroxide 3%, 6 to 8 cc. Wash and stain 2 to 3 minutes with a mixture of basic fuchsin, saturated alcoholic, 10 cc.; anilin oil (1 part) and 95% alcohol (3 parts) mixed, 5 cc.; distilled water, 30 cc.; acetic acid, 4%, 1 cc. Wash thoroly with water.

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If the cell treated with a chemical that inhibits DNA synthesis is treated to undergo the process of the cell cycle, the phase that will be affected is the S-phase of interphase.

<h3>What is interphase?</h3>

The interphase stage of cell division is the resting stage between two successive divisions.

The interphase is further grouped into three phases namely:

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The synthesis phase of interphase is the stage where new DNA strand is synthesized, hence, if the cell treated with a chemical that inhibits DNA synthesis is treated to undergo the process of the cell cycle, the phase that will be affected is the S-phase of interphase.

Learn more about interphase at: brainly.com/question/17485110

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