Definition
A technique which is used to separate, DNA, RNA or protein pieces from each other under the influence of electric field on the basis of their molecular size is known as gel electrophoresis.
Explanation
This method is very reliable for separation of large size molecule (over 1 million Da). Materials which are required for gel electrophoresis include:
1. TAE stock buffer
2. 1% agrose gel
3. Nucleic acid loading dye
4. Ethidium bromide
Procedure:
First prepare a stock solution of TAE buffer by adding appropriate amount of TAE in distilled water. then prepare 1% agrose by adding 1X TAE, some amount of agrose in water and heating it in microwve oven to mix them will. then pour agrose gel on tray and fix comb in it and keep it untill agrose dry. Then remove comb and pour some quantity of nucleic acid along with loading dye and ethidium bromide in each well. EtBr is used for staining nucleic acid.
When sample is poured in all well also pour reference marker in one well for comparison. now connect it with voltage for 30-35 min. After this take gel and see it under UV. a large number of nucleic acid pieces will be seen on gel under UV. those pieces which have small molecular weight will cover more distance compared to those having larger molecular weight.
Answer:
The given blank can be filled with acquisition.
Explanation:
An unconditioned stimulus provokes the unlearned response and an unconditioned response is the naturally taking place reaction. A neutral stimulus is illustrated as the stimulus that prior to conditioning do not generate any kind of response.
A type of learning wherein a subject begins to react towards neutral stimulus as it would perform with another stimulus by acquiring the path of associating the two stimuli is termed as classical conditioning.
An acquisition can be illustrated as the time of learning in classical conditioning where an individual begins to associate a neutral stimulus with an unconditioned stimulus that further permits the neutral stimulus to begin possessing the conditioned response.
I'm pretty sure that it's natural gas.
Answer:
The correct answers are B and C. Walter Sutton and Theodor Boveri were the first to suggest that growth of cancerous cells was a result of abnormal chromosomes.
Explanation:
The chromosomal theory of inheritance is a scientific theory that relates chromosomes with the transmission of inheritable characters. It is also called the chromosomal theory of Sutton and Boveri in honor of the two people who independently developed it in 1902, Theodor Boveri and Walter Sutton. This theory states that the alleles, the Mendelian genetic factors, are on chromosomes.
Theodor Boveri and Walter Sutton independently developed the chromosome theory in 1902, Boveri, studying embryonic development in sea urchin and Sutton in this work on meiosis in grasshopper.
Sutton and Boveri's proposition in 1902 that chromosomes are the factors of Mendelian inheritance was controversial until its demonstration in 1915 by the work of Thomas Hunt Morgan in the Drosophila melanogaster fly.
