Answer:
High concentration of glutathione should be included in the elution buffer for the given experiment to remove protein from the column.
Explanation:
Affinity chromatography is one of important biochemical technique of chromatography which depends on the affinity of ligand for the receptor.Here the ligand is a protein mixture which act as mobile phase and the receptor is present in the wall of chromatography column act as stationary phase.When the protein mixture is applied on the top of the column the substances present within the protein that have high affinity for the receptor present in the walls of chromatography column binds to the later but rest of the protein pass away through the column.High concentration of ligand is used within an buffer solution to remove the desired protein from the column.
From this point of view it can be stated that in the given question high concentration of glutathione should be used to remove the desired protein from the column.
A mixture is a compound of two or more substances a pure substance is only made of one kind of atom and a solution is composed of a liquid substance made of two or more types of atoms
Answer:
Gly-Ala
(Dipeptide).
Explanation:
A condensation reaction is a reaction in which two molecules combine to form a single molecule with the loss of a small molecule e.g water.
When two amino acids, such as alanine and glycine react, they yield a mixture of dipeptides (via dipeptide bond) and water. That is, The −OH from the carboxyl group (-COOH) of one amino acid combines with a hydrogen atom from the amine group (-NH2) of the other amino acid to produce water.
By increasing the size of cells
Answer:
K=1.93
Explanation:
Given reactions are:
A +2B ⇄ 2C K1=2.79
2C ⇄ D K2=0.186
D⇄ A+ 2B K=?
If we reverse the reaction then the euillibrium constant also become reciprocal of original value:
3rd reaction is a combination of reciprocal of 1st and 2nd reaction
2C⇄A +2B 1/K1;........................1
D ⇄ 2C 1/K2; ..............................2
On adding 1 and 2
D ⇄A +2B 
K=1.93
