2nd choice is the correct one
When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
Answer:
The correct answer is A. The region of the diencephalon that is responsible for maintaining homeostasis is the hypothalamus.
Explanation:
One of the main functions of the hypothalamus is to control the pituitary gland, an endocrine gland that participates in the regulation of various functions of the organism, many of them related to homeostasis (regulation of glycemia, osmolarity, etc).
Answer:
rain is very important for life in certain areas, this is why you see little life in deserts and even they need rain occasionally. more rain will lead to more life, first, it will be the plants that move in, then once they grow, vegetation eaters will move in, then after that meat-eaters will move in to eat those creatures and the ecosystem will flourish.