Answer:
c. Isolate bacterial plasmid
a. Select the gene of interest + insert into plasmid
f. Plasmid taken up by bacterium
d. Bacteria replicate
e. Cloned gene harvested from bacteria
b. Protein product harvested from bacteria
Explanation:
Genetic modification refers to the biological procedure involving the manipulation of an organism's genome in order to achieve a desirable result. This process is carried out by inserting or deleting a desirable gene (encodes the desirable trait) from the organism.
Bacteria DNA called PLASMID is commonly used for genetic engineering/modification procedure. The plasmid is used to convey/carry the gene into the organism. According to this question, the correct order of the procedure is as follows:
- c. Isolate bacterial plasmid
- a. Select the gene of interest + insert into plasmid
- f. Plasmid taken up by bacterium
- d. Bacteria replicate
- e. Cloned gene harvested from bacteria
- b. Protein product harvested from bacteria
