The material that are included in the hydrosphere that are not in liquid form are ice caps. So the correct option is A.
<h3>What is the hydrosphere?</h3>
The hydrosphere is the material formed by water that will be found on the earth and also in the earth's crust, usually it will change its form (liquid, solid, gaseous), forming the water cycle. This will allow life to be generated on the planet since it will regulate the climate in this way.
It will be made up of oceans, seas, rivers, lakes, snow, groundwater and ice.
Therefore, we can confirm that the correct option is A. ice caps.
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The answer would be d. the population has increased and so has the demand for energy sources
Thye can find ages or how long is been there for rocks
99% of water is reabsorbed into the blood when filteres at the bowmans capsule
Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
