Count the ones expressing the trait in the F2 gen. Divide by how many animals there are in F2. Times by 100.
Answer:
It depends on the size of the amplified DNA fragments (for example for SPAR markers consisting of 200 bp in length >> 1600 / 200 = 8)
Explanation:
The PCR based single primer amplification reaction (SPAR) methods are tools for detecting genetic diversity by using DNA markers such as Simple Sequence Repeats (ISSRs), Minisatellite DNA regions (DAMDs) and Random Amplified Polymorphic DNAs (RAPDs), which can be used alone or combined with each other. In the last years, the SPAR methods have gained attention in the scientific community because they are cost-effective and highly effective for the detection of both intraspecific and interspecific genetic variation. In the SPAR methods, the size of the bands will depend on the length of the amplified DNA fragments.
Flora:Calochortus flexuosus, Calochortus kennedyi, Opuntia echinocarpa.
Fauna:bighorn, coyote, bobcat, mountain lion, and mule deer. Mule deer
