Question

The ADG1(ADP glucose pyrophosphorylase 1) is involved in flowering timing in plants. You are interested in developing a DNA probe using partial protein sequence information. Part of the amino acid sequence is Glu-Glu-His-Asp-Val-Leu 1. determine the possible antisense oligo nucleotides possible from this amino acid sequence 2. How many total unique different oligonucleotide would need to be produced to cover all possibilities

Answer 1

Answer:

1)  UUC-AUG-AUC-AAG-UAA.

2) 48

Explanation:

1) Determine the possible antisense oligo nucleotides possible from this amino acid sequence

Given that the peptide sequence is = Glu-Glu-His-Asp-Val-Leu 1.

we will use a codon table to show the codons and Anticodons for each of the Amino acids  

Amino Acid   Codons                                                         Anticodons

 Glu                GAA, GAG                                                    UUC, CUC

 His                 CAU, CAC                                                    AUG, GUG

 Asp         GAU, GAC                                                    AUC, GUC

 Val                 GUU, GUC, GUA, GUG                             AAG, GAC, UAC,CAC

 Leu   UUA, UUG, CUU, CUC, CUA, CUG  | UAA, CAA, AAG, GAG,UAG,CAG

From the table above

The possible antisense oligo nucleotide sequence is : UUC-AUG-AUC-AAG-UAA.

2)  How many total unique different oligonucleotide would need to be produced to cover all possibilities

A desired peptide sequence requires 16 anticodons

Hence the total unique different oligonucleotide needed to be produced to cover all possibilities = 16 *3 = 48