Answer:
the diagram explains the process of DNA digestion and DNA ligation, which is usually used in molecular cloning techniques  
Explanation:
Molecular cloning can be defined as the process used to synthesize multiple copies of a particular DNA fragment. Molecular cloning requires the insertion of a foreign DNA fragment into an appropriate vector (e.g., a plasmid) through the action of specific enzymes that serve to cut and ligate DNA fragments. DNA digestion and DNA ligation use specific restriction enzymes and DNA ligases, respectively, in order to insert the foreign DNA fragment. For this purpose, restriction enzymes that generate single-stranded overhangs are preferred to create sticky ends which bind by complementary base pairing. Subsequently, a DNA ligase enzyme joins the DNA fragments together in order to create recombinant DNA molecules. DNA Ligation is often achieved by using a specific T4 DNA ligase, while there are many restriction enzymes that generate sticky-ends (e.g., BamHI, EcoRI, BaI228I, etc).
 
        
             
        
        
        
Answer: Proteins are made using DNA as a template. The DNA is turned into RNA, and the RNA is then turned into DNA. 
A change in these nucleotides could end up making some part of the protein different. A single nucleotide change could be silent (no change in the protein) or could change a single amino acid (amino acids are the building blocks of proteins). If that was an important amino acid, the protein might not function at all! A silent change can occur because the same set of nucleotides sometimes makes the same final amino acid (for example, reading "gcc" "gca" "gcg" or "gct" nucleotides all mean "alanine" amino acid). 
The deletion of a single nucleotide, or the addition of one, can change the entire sequence of amino acids that come after it! Nucleotides are read in sets of three, so this throws off how the DNA is read. If would be like turning "The brown fox jumps over the dog" into "The gbrow nfo xjump sove rth edo g". Completely different! All of the words are thrown off.
I know it is long but I hope it helped
:D
 
        
             
        
        
        
Methylphenidate refers to a CNS stimulant mainly used in the treatment of ADHD (attention deficit hyperactivity disorder) in children between the age of 6 to 12, adults up to the age of 65, and adolescents. It can also be used in the treatment of narcolepsy.  
However, the application of the drug may also bring some side-effects with it. Some of the common side-effects related to medicine are decreased appetite, headache, hallucinations, sweating, fever, dizziness, increased heart rate, weight loss, and others.  
In the given case, due to the application of the drug, the child is having hallucinations. Thus, in the given case, the nurse should recommend the client to report the manifestations as quick as possible, and discontinue the use of medicine.  
 
        
                    
             
        
        
        
Answer:
B I think sorry if I am wrong