One song is "On the Road Again" It represents that Lennie and George are traveling for a new beginning, working towards their dream and leaving the past behind.
Second song is "Way Out Here" It represents that they are the new guys and they are not welcomed with the others yet.
They have more wisdom and life experience, which makes them credible.
How the plot plays out can affect it because the plot can directly affect the theme because if the plot is someone stealing something and he gets caught then a theme could be stealing gets you know where. Hope this Helps :)