<span> Negative staining method is used to view viruses, bacteria, bacterial flagella, biological membrane structures, lipid aggregates and proteins or protein aggregates, which all have a low electron-scattering power. This method is used to view cells or certain organelles of cells more clearly under the microscope in the field of microbiology. </span>
<span>The tone, before conditioned to salivation, is simply a stimuli that the dog is responding to or ignoring in it's environment. Since it has no taught reactions to this sound, it is simply part of the environment the dog is in.</span>
2- Fault Lines (California is a big clue my homie)
Answer:
generally considered to have originated in the late nineteenth century as a reaction to the abuses of big business and the problems that plagued the lower socio-economic classes of that era.
Explanation:
