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3 years ago

15

A peak elutes from a column at 33.9 min, with a width at half height of 10.4s. How many theoretical plates does this column have

?

1 answer:

nadezda [96]3 years ago

8 0

Answer:

212724 plates

Explanation:

To solve this problem we use the formula:

N = 5.54 (<em>tr</em>/W₀.₅)²

Where

N is the number of theoretical plates,

<em>tr</em> is the retention time (33.9 min)

and W₀.₅ is the peak width at half height (10.4 s).

We <u>solve for N</u>:

10.4 s⇒ 10.4 / 60 = 0.173 min

N = 5.54 (33.9/0.173)²

N = 212724 plates

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