<span>The mitochondria in eukaryotic cells originated from prokaryotic cells, bacteria that were once free-living. The mitochondria have their own DNA, which is inherited from the mother in most species. Similarly, plant cells have organelles called plastids which contain their own DNA and originated from free-living bacteria. The chloroplasts in plant cells, which convert the energy of sunlight into chemical energy via photosynthesis, are a kind of plastid, and they originated from bacteria that could do photosynthesis.</span>
Answer and Explanation:
The steps for construction of the expression plasma
- STEP 1 : Introducing Notl and Hindlll confinement sites at the ends of the gene of interest by means of PCR
- STEP 2 :Digest the plasmid and DNA encoding gene with the Notl and Hindol confinement endonucleuses.
- STEP 3 : Ligate the cut plasmid and DNA fragment
- STEP 4 : Change the plasmid into E.coli for expression
- STEP 5 : Clean the protein by utilizing a column of immobilized

D1, A2, B3, C4, and sicnce 5 and E are left i guess they mach up, but it dont think that water is a wast.. but the other one r right
If I am understanding the question correctly your answer should be single-replacement reaction.
Hope this helps!
-Payshence xoxo
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