I think it is D.... hope i'm right
Answer:
A. pH
Explanation:
Denaturation of proteins is a protein whereby the protein molecule loses its primary structure and ultimately its physical functioning as a result of factors including pH, temperature or concentration of chemicals. Based on this definition, it is visible that denaturation of proteins can be caused by these three factors: pH, chemical and temperature.
However, in this case of PEPSIN enzyme, which is a proteinous molecule produced in the stomach of humans and functions in breaking down proteins, its denaturation occurs as it moves along the digestive tract to the intestines. This is as a result of the changes in pH in the digestive tract.
The pH of the stomach where pepsin is produced is acidic i.e. <3.0. However, as one moves towards the intestine, the pH increases, which is unfavorable or considered harsh for the pepsin enzyme. Hence, it gets denatured.
It depends on where your going and how many people are going to the trip. But in my opinion I think about 3 times because you need to make stops when you need to use the restroom and when your hungry.
Sorry if I am not right :(
I hope I helped you have a great day :)
<u>Answer</u>:
1. Type of medium - the nutrient availability is one of the vital factors for the growth of a bacterial colony. Thus, the used growth medium will fulfill this requirement.
2. Incubation temperature - all bacteria have an optimal temperature range in which maximum growth is achieved. Any variations from this will result in negative effects on the amount of bacteria that are produced.
3. Moisture - bacteria thrive in moist or wet environments. Thus, the availability of moisture is another vital factor for the growth of the colony.
