Answer:
the intensity of density dependent factors increase
The changes that one can expect to observe as a plant tissue culture develops is the development of new shoots and root.
<h3>What is a Culture?</h3>
This refers to the process in which microorganisms, living matter etc are grown in a specially prepared nutrient medium which are made up of different types of compounds.
In the case of a plant tissue culture, there will be new shoot and root formation due to the presence of nutrients in which the plants are exposed to for its growth and survival.
This is therefore makes changes in the shoot and root be the most appropriate choice.
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Answer: A- cell wall
A new cell wall forms between the two membranes of the cell plate.