Antibiotic and vitamins are examples of sac fungi True or false?

Intelligence tests are a good indicator of success in schools. A. True B. False

Viefleur [7K]

Answer:

according to me it is true

7 0

2 years ago

Design an experiment to answer the question, what is the effect of caffeine on the rate of mitosis in frog eggs? Label: hypothes

s2008m [1.1K]

Answer:

Explanation:

Null Hypothesis: Their will be no effect of the caffeine on the mitosis.

Hypothesis: Caffeine will inhibit the SHSY5Y cell division.

Dependent variable: Various concentration of the caffein compound.

Independent Variable: temperatures of the experimental condition. If their will be any effect of the temperature in mitosis then it will also affect the mitosis of control cells.

Experimental design.

Culture SHSY5Y neuronal cell line in the T-75 culture flask in CO2 incubator at 37 degree celsius. Next day, add cells from T-75 flask in the 24 well plate and allow cells to adhere to the well by incubating plate in CO2 incubator at 37 degree celsius. Add the compound caffeine at different concentration (say 1 microgram, 2 microgram, 3 microgram, 4 microgram) and incubate the plate for 24 hour. Determine the mitotic cells by following assay.

Assay to determine mitotic cell.

The reagent succinimidyl ester of carboxyfluorescein diacetate (CFSE) binds to cytoplasmic proteins. it is fluroscent reagent. And when cell divide due to mitosis, cytoplasm will also divide and hence, the protein which stained with the CFSE reagent will also goes in the daughter cell. Then, the fluorescence of the stained cells can be read on the plate reader.

Prepare the graph of the fluorescence values (Y-axis) verses different concentrations of the caffein (X-axis) and determine the slop of curve to know the rate of mitosis.

Following test and control samples will be used for the assay.

1. SHSY5Y cell line with added caffeine. It is test sample.

2. SHSY5Y cell line added with buffer. This important to know behaviour of cells under experimental conditions.

3. SHSY5Y cell line with solvent used to dissolve compound caffeine. It is important to know the toxicity or any effect of solvent on the mitosis.

5 0

2 years ago

How does ducks make their nests ?

Morgarella [4.7K]

Like any other bird, but only at the waterside.

5 0

3 years ago

HURRY HELP ME PLZ

Solnce55 [7]

Answer:

Explanation:

1. Describe two effects of insecticide use on the forest ecosystem other than the intended reduction of moths to protect the trees.

1. Insecticides when sprayed on a certain areas or spots can be blown by the wind to other areas or flow with rain water into nearby streams or local water bodies or seep through the soil into ground water. This is called indirect application or secondary poisoning,

2. Insecticides can affect the food chain because they reduce the biomass of primary consumers and there is is less food available to secondary consumers which eat poisoned plants or prey those who have been exposed to pesticides.

Insecticides can travel great distances through the environment and they can also flow with rain water into streams so fish can be directly or indirectly impacted by pesticides. The phenomenon of nutrient enrichment of aquatic bodies is known as eutrophication, which deteriorate the water quality leading to death of fish. Some long-term exposures cause abnormalities or mutations in developing fish larvae, while acute exposure can cause immediate fish die-offs. The liver, kidney, brain and gills of exposed fish are extremely vulnerable to chemical exposure.

6 0

3 years ago

A C4 plant like corn or sugarcane would not thrive in a cool shady forest because of:________

Alborosie

Answer:

The correct answer is - B. lower environmental availability of CO2

Explanation:

In C4 plants they have developed a mechanism for the CO2 concentrating and leads to the lower environmental availability of CO2 for these plants, C4 to avoid photorespiration.

These plants tend to be found in drier environments with high sunlight levels and not thrive in a cool shady forest due to this mechanism that results in low availability of carbon dioxide.

6 0

3 years ago