A chemical bond to form in a compound must be formed my two or more atoms attracted to each other.
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Cation-exchange chromatography is used when the molecule of interest is positively charged, the stationary phase is negatively charged and positively charged molecules are loaded to be attracted to it. So, the amino acids with negative charge will elute the first. Glutamate, leucin, arginine is the order of elution because of their pI values ~3, ~6 ~10.
The answer is 60% are active
Genetic drift scientific explanarions are based on empirical data and evidence
