Answer:
Extraction of genomic DNA
Explanation:
Resuspend the cells(any number), smaller numbers also work, in 10-20 ul of TE buffer or milli Q water. boil for 5-10 min in a waterbath, quick chill, short spin. use the supernatant as a template for PCR.
If cells are very less, u can directly add cells to the PCR reaction without enzyme,boil for 5-10 min in a waterbath, let it cool slowly, short spin. Add enzyme to the supernatant and start the reaction at extension step followed by normal PCR reaction steps.
There numerous of them but some of them would be contaminated soil which can be followed with altered soil chemistry that contains fewer soil organisms that are essential. The third one would be surface runoff due to extensively modified hydrology of the area.
The other answer is wrong. It would be pollution. Hope this helps you!
I would say down wind side but I'm not 100% sure
Answer:
cytoplasm
Explanation:
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