The right option is; dark-field microscope
A light microscope that makes the specimen appear light on a dark background is called a dark field microscope.
Dark field microscopes are light microscopes that are used in different ways to clearly view various specimens that are unstained, transparent, and hard to see using a light field unit. Dark field microscopes are very effective because they show the details of unstained and live samples. It is also very simple to use, and inexpensive to set up.
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In eukaryotic cells, which have a nucleus, the cytoplasm is everything between the plasma membrane and the nuclear envelope.
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either close to the shore or deeper out into the sea
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