When it is winter in the Northern Hemisphere, the Southern Hemisphere faces the Sun more directly and thus experiences warmer temperatures than the Northern Hemisphere. Meaning that the date is June 21- summer
When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
Explanation:
in the circumstances in which the attempts do not contravene the ethical and judicial laws.
