Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
B.produce antibodies when triggered by antigens
Answer:
Metabolism refers to all the chemical reactions in the body, including those that use oxygen and create carbon dioxide. Oxygen and carbon dioxide, therefore, are involved in both respiration and metabolism. Metabolic reactions are sometimes referred to as cellular respiration, which can cause confusion.
Because you could easily contaminate the results of the urine if you don't follow exactly what the strict rules dictate This could be a very dangerous thing because if you contaminate the results, then other doctors would think that the patient has some disease that needs to be taken care of which in reality they do not. The patient could also have to go through things or waste money on things that they don't need to because there was something in the urine that wasn't supposed to be there which was added because they didn't follow the strict rules. Then the doctor who contaminated it could be sued for malpractice which is never a good thing.
