Answer:
The correct answer is: d. REs activity is best preserved by freezing the RE and thawing it out for brief periods of time as needed.
Explanation:
- Restriction enzymes can be defined as endonucleases that can recognize specific nucleotide sequence in a DNA (Deoxyribonucleic acid) sequence and cause breakage of phosphodiester bond in the DNA at the target site. Hence, it has the capacity of cleaving a DNA sequence.
- These are made up of proteins which are required to be present in the accurately folded conformation in order to function.
- Any form of alteration in the three-dimensional structure of the protein induced due to physical, chemical, mechanical or physiological factors can cause denaturation of the protein which in turn can render the protein non-functional.
- Freezing of restriction enzymes (REs) directly without the use of glycerol will cause the movement of water molecules within the proteins as well layering of water molecules on the protein surface.
- At low temperature these water molecules change to ice crystals which can alter the conformation of the protein or can cause breakage of peptide bonds in the proteins by physical force.
- This can cause the protein to get denatured and hence non-functional.
- Hence, it is never advisable to preserve REs by freezing directly without glycerol.
- Glycerol forms a layer around the protein molecules and prevents the protein molecules from coming into direct contact with the water molecules and hence prevents protein denaturation.
Answer:
There is no cure for cystic fibrosis, but treatment can ease symptoms and reduce complications.
Explanation:
1). For those with cystic fibrosis who have certain gene mutations, doctors may recommend a newer medication called ivacaftor. This medication may improve lung function and weight, increases the activity of Cystic fibrosis transmembrane conductance regulator (CFTR)protein and reduce the amount of salt in sweat. It has been approved by the Food and Drug Administration for people with cystic fibrosis who are age 6 and older. The dose depends on your weight and age.
2). For people with a certain gene mutation who are age 12 and older, another drug is available that combines ivacaftor with a medication called lumacaftor. This drug is called orkambi.
The use of Orkambi may improve lung function and reduce the risk of exacerbations.
I hope you're clear on this Daxxy
TRUE. the placenta develops in the mother's uterus during pregnancy. The structure provides oxygen and nutrients to the growing baby and removes waste products from the babys blood
Olivine, pyroxene, amphibole, mica, and quartz
Answer:
The correct answer is D. is a facultative anaerobe
Explanation:
A facultative anaerobe is the organisms that can live in the absence of oxygen but prefer to utilize the oxygen when oxygen is present in the environment.
An anaerobic organism requires more amount of energy sources like carbohydrates because they use the fermentation process to make energy and this process results in the formation of less energy than aerobic respiration.
All the bacteria of family Enterobacteriaceae that live in the intestine are facultative anaerobes. Therefore facultative anaerobe can thrive in both the presence and absence of oxygen so the correct answer is D. is a facultative anaerobe.
