Answer:
In this study, we tested the effects of NEAA-deprived diets and checkpoint inhibitor anti-PD-1 and anti-PD-L1 in colon cancer using syngeneic mouse model (Balb/c) bearing tumors of mouse colorectal cancer cell line CT-26. Three diets were tested, including a natural rodent diet Teklad ENVIGO Global 16% Protein Rodent Diet (control 1), a formulated NEAA-complete diet COMPLETE (control 2, using amino acid mix in place of protein), and a formulated NEAA-deprived diet FTN203 (treatment, using amino acid mix in place of protein). Both COMPLETE and FTN203 have the same nutritional structures, contain 17% w/w protein equivalent, and are isocaloric. After tumor size-based randomization, these diets were provided to mice ad libitum throughout the whole test. Each of these diets was used alone or combined with anti-PD-1 antibody (i.p., twice per week for 2 weeks) or anti-PD-L1 antibody (i.v., twice per week for 2 weeks).
Explanation:
The ecological footprint is a general assessement of the population while the ecological footprint per capita means the ratio a country's per capita Footprint to the per capita biological capacity available on Earth.
<h3>What is an Ecological Footprint?</h3>
This is best defined as the impact of human activities which is measured in terms of the area of biologically productive land and water required to produce the goods consumed and to assimilate the wastes generated.
The importance of Ecological Footprint is due to the fact that it does measures the biologically productive area that are needed to provide for everything that people demand from nature such as fruits, vegetables, meat, fish, wood, cotton, fibres, as well as absorption of carbon dioxide from fossil fuel burning and space for buildings and road.
In essence, the ecological Footprint assess how fast or quick the people consume resources and generate waste compared to how fast nature can absorb our waste and generate resources.
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In a way, this is a double edged sword. On one hand, the animals are needed to best test the effects, but on the other hand it can be seen as very unethical. Honestly, I don't really see a way around using the animals. Unless you get human volunteers, but there is no way to fully make them understand what they are getting themselves into which can turn unethical very, very fast. If all of a sudden one of your human volunteers decides that they do not want to be a part of the test anymore, there is noting you can do to reverse what has already been done.
Past and light because it takes a minute for light to get from one place to another
