A rapid lateral flow immunoassay is presented that uses carboxyl-modified superparamagnetic nanoparticles as labels that can be quantified by highly sensitive multi-channel electronic readers. The approach is generic in that it is likely to be applicable to numerous small molecules. The method permits both single- and multiplex assays at a point-of-need without sample pretreatment. It is user-friendly and offers attractive characteristics demonstrated here for detection of morphine, fentanyl and methamphetamine in . The competitive immunoassay uses commercially available reagents that do not require special permissions. After migration of sample, the lateral flow test strips are subjected to an alternating magnetic field at two frequencies. The response from the nanolabels is readout at a combinatorial frequency from the entire volume of a porous immunochromatographic membrane by the magnetic particle quantification technique.
application to the determination of drugs of abuse.
Estimating the detection time of a drug in urine is complex because of many different influencing factors and the lack of experimental data. Detection times vary depending on dose and route of administration, metabolism and characteristics of the screening and confirmation assays. Using a cut-off value of 1000 ng/mL, urinary samples can be positive for amphetamine for up to 5 days after intake of the drug.
Answer:
genes containing dna.
Explanation:
during reproduction genes containing dna, are passed along to an organisms offspring. these genes ensure that the offspring will belong to the same species.
Compounds consist of atoms of different elements. In compound the atoms are present in fixed proportion. By changing the proportion compound will changed. For example,
Nitrogen and oxygen reacted to form the compound. By changing the proportion both will form different compound.
NO₂
Nitrogen dioxide consist of one atom of nitrogen and two atoms of oxygen. Their ration is 1 :2
NO
In nitric oxide one atom of oxygen and one atom of nitrogen are present.
Their ratio is 1 : 1.
N₂O
Nitrous oxide consist of two nitrogen atoms and one oxygen. Their ratio is
2 : 1
N₂O₄
Dinitrogen tetroxide consist of two nitrogen atoms and four oxygen atoms. Their ratio will written as 2 : 4
N₂O₅
Dinitrogen pentoxide consist of two nitrogen atoms and five oxygen atoms. Their ratio will written as 2 : 5
The answer is Sodium Chloride
Answer:
Climate change is rapidly becoming known as a tangible issue that must be addressed to avoid major environmental consequences in the future. Recent change in public opinion has been caused by the physical signs of climate change–melting glaciers, rising sea levels, more severe storm and drought events, and hotter average global temperatures annually. Transportation is a major contributor of carbon dioxide (CO2) and other greenhouse gas emissions from human activity, accounting for approximately 14 percent of total anthropogenic emissions globally and about 27 percent in the U.S.
Fortunately, transportation technologies and strategies are emerging that can help to meet the climate challenge. These include automotive and fuel technologies, intelligent transportation systems (ITS), and mobility management strategies that can reduce the demand for private vehicles. While the climate change benefits of innovative engine and vehicle technologies are relatively well understood, there are fewer studies available on the energy and emission impacts of ITS and mobility management strategies. In the future, ITS and mobility management will likely play a greater role in reducing fuel consumption. Studies are often based on simulation models, scenario analysis, and limited deployment experience. Thus, more research is needed to quantify potential impacts. Of the nine ITS technologies examined, traffic signal control, electronic toll collection, bus rapid transit, and traveler information have been deployed more widely and demonstrated positive impacts (but often on a limited basis). Mobility management approaches that have established the greatest CO2 reduction potential, to date, include road pricing policies (congestion and cordon) and carsharing (short-term auto access). Other approaches have also indicated CO2 reduction potential including: low-speed modes, integrated regional smart cards, park-and-ride facilities, parking cash out, smart growth, telecommuting, and carpooling.
Explanation: