Answer:
-use PCR to amplify the gene for GFP
-perform a restriction digestion of the GFP gene and the plasmid
-ligate together the GFP gene and the plasmid to generate a recombinant plasmid
-transform bacteria with the recombinant plasmid using electroporation
-plate the bacterial cells, and screen for positive transformants
Answer:
its a sign of your body's natural fight against an affection
Explanation:
hope this helps:)
The circulatory system brings nourishment to all cells of the body. The circulatory system exchanges critical gases with the respiratory system; unneeded carbon dioxide is exchanged for much needed oxygen. The respiratory system provides oxygen so the body can "burn" (oxidize) the food that is brought into the body through the digestive system<span>.</span>
Superantigens cause the stimulation of T cells to overproduce cytokines.
- Some pathogens in an unusual way take over the immune system and mislead it by interfering in the adaptive immune system.
- Instead of processing by APC (Antigen Presenting Cells), superantigen is directly bonded to MHC-II (Major histocompatibility complex) and TCR (T cell receptor) for a longer period than normal.
- As a result, about 20% of T cells are stimulated to produce cytokines such as interleukin-2 (IL-2), interferon-γ (INF-γ), and tumor necrosis factor α (TNF-α) known as Cytokine storm. Thereby, affected individuals experience some adverse symptoms like toxic shock syndrome and rheumatic fever.
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This could be the different times you did the experiment
