Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
1. A transgenic organism expresses DNA that has been derived from another species. These organisms have altered genomes and are generated in the laboratory for the purposes of research, for example, the "knock-out" mice are tansgenic mice with a particular gene of interest disabled.
2. Recombinant DNA is the DNA that has been altered to contain genes, or portions of genes, from different organisms. The DNA molecules are formed in the lab using recombination methods to bring together genetic material from multiple sources and therefore creating sequences that are not found in the genome.
3. Restriction enzymes are found naturally in bacteria and they cut DNA at specific nucleotide sequences. These enzymes are also called restriction endonucleases, they cleave DNA into fragments at or near specific recognition sites called restriction sites. They are found in bacteria and archaea and they serve to provide a defense mechanism against invading viruses.
4. Biotechnology is the use or alteration of organisms, cells, or biological molecules to produce food, drugs, or other goods. Living systems and organisms are used to make or develop products. Biotechnology enhances cellular and biomolecular processes to develop technologies and product for the purposes of improving life and health.
5. Polymerase chain reaction is a technique that is used to quickly synthesize billions of copiies of a specific segment of DNA. It is a quick and a very efficient way to generate many copies of DNA. The process is applicable when there are small quantities of DNA to analyze. The events of PCR involves; separation of DNA strands, addition of primers, use of DNA polymerase to produce second strand of DNA.
6. In the process known as transformation, bacteria take up pieces of DNA from the surroundings. Bacterial transformation results from the uptake of naked DNA (DNA without associated cells or proteins). Transformation may also occur naturally in some species of bacteria.
<span>Blockage of the Cerebral Artery can reduce blood supply to the brain, causing a stroke (Ishcemic Stroke)
Blood is carried to the brain by two pair of arteries, i) The Internal Carotid arteries, ii) The Vertebral arteries.
Stroke is a neurovascular condition affecting blood vessels in the brain. There are two types of Stroke i) Ischemic Stroke ii) Hemorrhagic Stroke.
In Ischemic stroke occlusion of a cerebral artery causes the damage to brain tissue dependent on blood supply from the affected vessel</span>
<span>This principle, or thought process, is that in basic sports fitness programs, all athletes training to continuously improve must always work harder an harder as their bodies become more used to their existing work out routines.</span>
I believe the correct answer is D. <span>ears pinned back, eyes averted or lowered, and lips pulled back and down.
When dogs are afraid, their ears are lowered to show fear because of something they may have done wrong. They are also scared or embarrassed to look at their owner because they know they will receive some sort of punishment. Their tail is also usually tucked between their legs to show they are afraid.
</span>
