The correct option is 3.
Enzymes are biological catalysts which speed up the rate of biochemical reactions inside the cells. Enzymes are very specific in action and they require specific conditions called optimum conditions for them to function optimally.
When the optimum conditions are not met, an enzyme's activity will be lowered or totally stopped. 
Conditions that affect the optimum activity of an enzyme include: temperature, pH level and regulatory activity of regulatory enzymes and hormones.
        
             
        
        
        
Nitrogenous base DNA consists of four unique nucleotides that each contain one unique nitrogenous base—adenine (A), thymine (T), cytosine (C), or guanine (G).
The specific arrangement of these four bases within the DNA of each organism gives that organism its unique traits; here are the arrangements:
-<u>Adenine</u> is paired with <u>Thymine</u> (think of A for apple and T for tree)
-<u>Cytosine</u> is paired with <u>Guanine</u> (think of C for car and G for garage)
search "DNA base pairs" and go to images for better understanding