1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
drek231 [11]
2 years ago
15

You first use PCR to amplify the fragment so that there is sufficient DNA for sequencing. You carry out dideoxy sequencing and t

hen separate the products of the sequencing reactions by gel electrophoresis. Draw the bands that should appear on the gel from the four sequencing reactions.
Biology
1 answer:
dsp732 years ago
3 0

Answer:

The first attached figure below shows the design of an agarose gel with four sequencing reactions. The second figure presents a photo of an agarose gel, so that you can better understand how the bands are represented in this gel.

Explanation:

To view the bands of four sequencing reactions on an agarose gel, you will need to use a melted agarose gel, plastic combs suitable for that reaction and a container suitable for that type of gel. You will place the plastic combs in the container and pour all the gel into the vat and wait for the melted gel to solidify. The plastic combs will form holes in the hardened gel where the DNA samples will be placed.

Once the gel is hardened, you will remove the plastic combs and begin to apply the sequenced DNA.

The sequenced DNA samples will be mixed with a dye, usually bromophenol blue, which will allow you to visualize the bands formed on the gel. You will also apply the dye to a sample without DNA containing only the dye, which serves as a comparison for the size of the bands.

Each sample of DNA will be plated in the column of holes formed by the plastic combs. Then, this container, with the gel, will be placed in a larger container that contains a loading buffer. The larger container will be closed and an electric field will be applied that will force the DNA samples to be moved from one pole to another inside the container, in this case, the samples leave the negative pole for the positive pole.

After a few minutes, it is possible to visualize the DNA displacement and at the end of the procedure it will be possible to visualize the formation of bands as shown in the drawing and in the figure below. The size of these bands can be compared and analyzed.

You might be interested in
Why do you think scientists track the genes of other organisms such as rats or mice in the database? Do you think rat genes are
DENIUS [597]

Explanation:

Understanding through into genetic risk factors for various illnesses in the human population come from mouse research. Manipulation of the mouse genome is quite simple, for example, adding or deleting genes to better understand their function in the body.

The majority of mice and rats used throughout medical studies are inbred, which means they are genetically virtually similar, making the outcomes of medical trials more consistent.

6 0
2 years ago
If yall dont know a answer and give the wrong answer, what are you going to to?
oksano4ka [1.4K]

Answer:

try to solve it on a paper

3 0
1 year ago
What cells do flu viruses infect?
k0ka [10]
They mainly target airway epithelial cells
6 0
2 years ago
Which explains what happens to a warm air mass at a cold front? A. A warm air mass overtakes a cold one, and the warm air rapidl
sergejj [24]
<span>The answer for your question is
B. A warm air mass overtakes a cold one, and the warm air gradually rises and cools. The water in the air condenses to form clouds and gentle rain.</span>
6 0
3 years ago
Why can't science prove something beyond a shadow of a doubt?
jarptica [38.1K]

Answer:

Scientific theories can never be proven true beyond all doubt; they can only be supported by a wide body of evidence. Only one of the statements that follow uses the term theory in its correct, scientific sense.

Explanation:

6 0
2 years ago
Read 2 more answers
Other questions:
  • [BWS.02]How does science help in the progress of the society?
    6·1 answer
  • Are there programs and research currently in place to save the endangered polar bear population?
    15·1 answer
  • Which of the following is a natural resource with a limited quantity that is consumed must faster than it can be replaced? A. hy
    9·2 answers
  • The weight of a substance is defined as its mass divided by its volume.
    6·1 answer
  • A medicine to remove the redness in eyes was tested in a group of 100 students. Each student took either the medicine or a place
    15·1 answer
  • The stretch hold is a restraint technique:
    15·1 answer
  • It has been theorized that the organelles of eukaryotic cells evolved from prokaryotes living symbiotically within a larger cell
    9·1 answer
  • Where can you find carbon?
    14·2 answers
  • How will win in a fight pikachu are sonic
    11·2 answers
  • During asexual reproduction yeast cells can produce _____
    12·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!