Answer:
D.
Explanation:
A 'common ancestor' on a cladogram is all of those points where your lines come down and meet the primary line at the bottom.
In this case, your most recent common ancestor is at the point connected to the crocodile. All species at or past that point have that common ancestor, which include only the crocodile and the bird.
When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
Answer:
Extraction of genomic DNA
Explanation:
Resuspend the cells(any number), smaller numbers also work, in 10-20 ul of TE buffer or milli Q water. boil for 5-10 min in a waterbath, quick chill, short spin. use the supernatant as a template for PCR.
If cells are very less, u can directly add cells to the PCR reaction without enzyme,boil for 5-10 min in a waterbath, let it cool slowly, short spin. Add enzyme to the supernatant and start the reaction at extension step followed by normal PCR reaction steps.
The answers A. C. D. are all physical reactions
The answer is B. Digestion of food
When food is digested it is chemically broken down
Hope this help!
