Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
ADP (Adenosine diphosphate; di= two)
Explanation:
When the terminal (third) phosphate is cut loose, ATP becomes ADP (Adenosine diphosphate; di= two), and the stored energy is released for some biological process to utilize.
<span>The ventricles are the part of the heart that are responsible for the pumping action to move blood throughout the body. Therefore, the muscles in the ventricles are much larger and thicker to do their job properly. The atrium only has to pump blood through an open valve (the tricuspid valve) into the right ventricle. There is much less resistance to this flow of blood so much less muscle is needed. </span>
Answer:
In mendelian inheritance, the alleles for a gene show normal dominant-recessive relationship. Chromosomes also show crossover due to which new random combination of traits is possible in the offspring. This crossover takes place between homologous chromosomes during meiosis I.
Organellar DNA like the ones present in mitochondria and chloroplast do not follow mendelian inheritance because unlike nuclear chromosomes they do not have cross over events. There is no orderly segregation of alleles during meiosis. Traits controlled by them are usually inherited as it is and usually it is from the maternal parent because paternal gamete like sperm does not contain mitochondria.
